The cell imaging and high content analysis system CQ1 is a fully integrated desktop imaging system. It uses microlens-enhanced dual Nipkow scanning unit containing about 20,000 pinholes and a second spinning disk containing the same number of microlens to focus excitation laser light into each corresponding pinhole are mechanically fixed with a motor and raster, simultaneously scan the field of view with 1,000 laser beams when spinning with the speed up to 4,500 rpm. Multi-beam scanning means not only higher scanning speed, buy also significant lower photobleaching and phototoxicity, since multiple excitation acquires only a very low level of laser power on the surface of specimen.
CQ1 enables 3D imaging and quantification of live cell clusters, such as spheroids within a 3D culture vessel, as they are, keeping the cells intact. CQ1 exports feature data in general formats which are readable by various third-party software for advanced data analysis. It is possible to construct fully customized CQ1-based system by integrating with external systems, via robot for culture dish handling. With the help of novel probes, CQ1 will have a more efficient imaging and analysis function.