The CellVoyagerTMCV1000 Confocal Scanner Box is a fully integrated desktop imaging system. With
its microlens enhanced dual Nipkow disc scanning technology, phototoxicity and
photobleaching are drastically reduced, making it ideal for use in observing highly
delicate life processes such as iPS/ES cell generation and embryogenesis. The
system is easy to use and eliminates the need for a dark room.
A Nipkow spinning disk containing about 20,000 pinholes and a second spinning disk containing the same number of microlens to focus excitation laser light into each corresponding pinhole are mechanically fixed with a motor, and very rapidly raster scan the field of view with about 1,000 laser beams when rotated. Multi-beam scanning with the CSU not only increases scanning speed, but also results in significantly lower photo bleaching and photo toxicity, because multiple excitation needs only a low level of laser power at the specimen to fully excite fluorescence. More than 1,500 units of the CSU series are used as the de facto standard tool for live cell imaging, worldwide .